Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 1. PDL1 expression in cancer and noncancer cell lines. A, relative expression of PDL1 from total RNA isolated from cell lines. B, representative PDL1 protein expression levels in corresponding high- and low- expressing cell lines by IHC. C, representative PDL1 protein expression levels in corresponding high- and low- expressing cell lines by flow cytometry.

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing, Isolation, Cytometry

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 2. Relative expression of PDL1 in 38 osteosarcoma specimens. PDL1 expression was evaluated from total RNA by quantitative real-time RT-PCR and showed that the expression levels range over 4 log (5,000-fold).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing, Quantitative RT-PCR

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 3. Overall survival of 37 patients with osteosarcoma in relation to PDL1 gene expression. The median overall survival for PDL1-low patients was 89 months compared with 28 months for PDL1-high patients, which showed a trend but was not statistically significant (P ¼ 0.0544).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Gene Expression

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 4. Correlation between PDL1 gene expression and TILs by IHC. A, representative TILs in osteosarcoma tissues (400); score 0, no TILs; 1, rare/few TILs; 2, brisk/prominent TILs. B, significant positive correlation was shown between PDL1 gene expression and TILs in patients with osteosarcoma (P ¼ 0.0117).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Gene Expression

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 5. Characterization of the origins of metastases. A, PDL1 expression is significantly higher in metastatic osteosarcoma tumors that originate from lung than from other locations (P ¼ 0.0024). B, TILs also exhibit a positive correlation with pulmonary osteosarcoma metastasis compared with nonpulmonary metastasis (P ¼ 0.0443).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing

Journal: Clinical and Experimental Immunology

Article Title: The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring

doi: 10.1093/cei/uxac086

Figure Lengend Snippet: the levels of ENO1Ab in the H-ENO1Ab dams and their pups. Serum and amniotic fluid samples were collected as depicted in . Anti-ENO1 total IgG and its subtypes were measured by enzyme-linked immunosorbent assay (ELISA). The results are shown above for the dams (A, n = 6-14/group, ** P < 0.01, *** P < 0.001 vs. the control group) and the pups (B, n = 14-19 for total IgG, n = 5-8 for IgG1, n = 5-9 for IgG2a, IgG2b and IgG3, ** P < 0.01, *** P < 0.001, not significantly (NS) vs. the pups of the control group).

Article Snippet: After 2 h incubation at RT followed by repeated washing, the plates were further incubated with diluted horseradish peroxidase (HRP)-labeled goat anti-mouse total IgG (1:200 diluted for TgAb;1:10 000 for ENO1Ab), IgG1(1:1000 for ENO1Ab), IgG2a (1:1000 for ENO1Ab), IgG2b (1:1000 for ENO1Ab), or IgG3 antibodies (1:1000 for ENO1Ab), which were all from Bethyl (USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Clinical and Experimental Immunology

Article Title: The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring

doi: 10.1093/cei/uxac086

Figure Lengend Snippet: IgG deposition and the distribution of C3, MAC, and CD16 in the brain tissue of the pups from H-ENO1Ab dams. All the pups were obtained as depicted in . (A) Representative images of IgG deposition in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 and P40 ( n = 4/group) after immunofluorescence (IF) staining with FITC-conjugated goat anti-mouse IgG (green, ×200). (B) Representative images for the colocalization of IgG and ENO1, NeuN, and CD34 in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 ( n = 4/group) after double IF staining with FITC-conjugated goat anti-mouse IgG (green) and rabbit anti-ENO1 and NeuN, rat anti-CD34 (red, ×200). (C and E) Representative images of the colocalization of IgG and C3 and CD16 in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 and P40 ( n = 4/group) after double IF staining with FITC-conjugated goat anti-mouse IgG (green) and rabbit anti-C3 and CD16 (red, ×200). (D) Representative images of MAC expression in the brain tissues from the pups of H-ENO1Ab dams and the those of the control group on P10 and P40 ( n = 4/group) after IF staining with mouse anti-C5b-9 (green, ×200). Nuclei were counterstained with DAPI (blue).

Article Snippet: After 2 h incubation at RT followed by repeated washing, the plates were further incubated with diluted horseradish peroxidase (HRP)-labeled goat anti-mouse total IgG (1:200 diluted for TgAb;1:10 000 for ENO1Ab), IgG1(1:1000 for ENO1Ab), IgG2a (1:1000 for ENO1Ab), IgG2b (1:1000 for ENO1Ab), or IgG3 antibodies (1:1000 for ENO1Ab), which were all from Bethyl (USA).

Techniques: Control, Immunofluorescence, Staining, Expressing

Journal: Toxicology Reports

Article Title: Nevada desert dust with heavy metals suppresses IgM antibody production

doi: 10.1016/j.toxrep.2018.01.006

Figure Lengend Snippet: Autoantibody production against (A) myelin basic protein (IgM and IgG) or (B) glial fibrillary acidic protein (IgM and IgG) in adult female B6C3F1 mice following oropharyngeal aspiration exposure to dust samples from NDRA CBN 6. Data are presented as mean ± standard deviation. Sample size for each group was 5–6 animals. The (*) indicates a response statistically different from the 0 mg/kg group (p < 0.05).

Article Snippet: Monoclonal antibodies coupled to fluorochromes specific for the following markers were used at a concentration of 1 μg/10 6 cells: anti-mouse CD25-FITC, rat IgG1-PE isotype control, rat IgG2b-AF647 isotype control, and rat IgG2b-FITC isotype control (BD Pharmingen, San Diego, CA, USA).

Techniques: Standard Deviation